High performance liquid chromatography (HPLC)

High performance liquid chromatography (HPLC) is an analytical technique used to separate molecules from a complex mixture in a column. Once the molecules have been separated, they enter a detector. To quantify molecules, this device has conventional detectors such as a UV-Visible spectrometer (DAD diode Array detector), a fluorescence detector as well as refractive index detectors (RID). By applying these detectors, a wide range of molecules such as sugars, pharmaceutical molecules, polyphenols could be quantified. Moreover, the size of molecules, such as lignin could be determined.